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Data of Dissertation


Author: Carolina Tolomini Miranda

Títle: Variation of nutritional conditions for growth optimization and lipid production by microalgae

Year: 2017.                                         Full text (in Portuguese)


Culture medium optimization is a crucial step for viability of biodiesel production from lipids of microalgae. In this work were evaluated the effects of nutrients limitation, salinity increase and CO2 addition in lipid production by two strains of Chlorophyceae microalgae, Ankistrodesmus sp. (ANRF-01) e Chlamydomonas sp. (CHLRN-01), to determine the optimal condition for these production. The results revealed that the reduction to 75% of ASM-1 nitrate and phosphate concentrations presented superior results of biomass and lipid production, in order of 23,0 g.L-1 and 31,4% for ANRF-1 and 60 g.L-1 and 22.5% for CHLRN-1, respectively. Both strains were suitable for cultivation in brackish medium containing 0,5g L-1 NaCl. ANRF-1 showed an expressive increase in biomass production and lipid yield in ASM-1 medium 5% CO2 added, showing an important potential for CO2 mitigation from combustion gases. In other hand, for CHLRN-1 the 5% CO2 addition leaded to an inhibitory effect on cell growth with a decrease in lipid production. Being therefore the addition of 5% CO2 not recommended for this strain. The Fatty acid profile produced by ANRF-1 and CHLRN-1 was mainly composed of C16:0 (palmitic acid), C18:1n-9c (oleic acid), 18:2n-6c (linolenic acid) and C18:3n-3 (α-linolenic acid) promising not for biofuels industry only, but also for the pharmaceutical and food industries, due to the high variety of polyunsaturated fatty acids of omega3 and omega 6.